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How to read a certificate of purity

Batch number, method, identity and purity: what each line on a certificate of purity tells you, and how to match it to the vial on your bench.

How to read a certificate of purity – NEXCELL Journal

Every NEXCELL batch is identified by a batch number printed on the label. A certificate of purity is the document that ties that number to an independent analytical result. Knowing how to read one takes a couple of minutes and saves a lot of second-guessing later.

1. Start with the batch number

The batch number on the certificate must match the one on your vial or pen exactly, for example NX-BPC-2609-A. The first letters identify the compound, the four digits are the production year and month, and the final letter separates batches made in the same month. If the numbers don’t match, the certificate doesn’t describe the product in front of you.

2. Check the method

Most certificates list two techniques:

  • HPLC-UV separates the sample into its components and measures how much of the signal belongs to the target compound. This gives the purity figure.
  • Mass spectrometry (ESI-MS) measures the molecular mass, confirming the compound is what the label says. This is the identity check.

Purity without identity tells you a sample is clean, not that it is the right molecule. You want both.

3. Read the purity figure in context

Purity is reported as a percentage of the HPLC signal, for example 99.4%. It is a relative measure: it describes the proportion of the main peak, not the absolute amount in the vial. The stated amount (for example 5 mg) is a separate specification.

Line on the certificateWhat it tells you
BatchWhich production run the result belongs to
TestedThe date the sample was analysed
MethodHow purity and identity were measured
IdentityWhether the measured mass matches the compound
Purity (HPLC)Share of the signal from the target compound

4. Look at the dates

The test date should sit close to the production date in the batch number. A certificate from a much older batch is not evidence for a newer one.

Finding the certificate for your batch

Our certificate library lists each batch with its test date and purity. Search by compound or batch number, and if a certificate isn’t published yet, use the Request button on that row and we’ll email it to you, usually within one working day.

For research purposes only. NEXCELL products are not medicines and are not intended for human consumption, diagnosis, treatment, cure or prevention of disease.

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